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KMID : 0368419910340020121
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Journal of Plant Biology
1991 Volume.34 No. 2 p.121 ~ p.127
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Changes in Nodule-Specific Proteins during Nodule Development oi Canavalia lineata
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Abstract
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Total soluble proteins from three developmental stages of induced root nodules of Canavalia lineata were compared with those of non-nodulated roots by SDS-PAGE and two dimensional (2-D) gel electrophoresis. Thirteen nodule-specific protein (nodulin) bands were identified by the former and 30 nodule specific protein spots were detected by the latter method respectively. Some of the nodulins were detected differentially depending on the nodule´s developmental stages. For example, only three leghemoglobin (Lb)-like protein spots appeared at stage Ⅰ (d＜2 ㎜), but two additional Lb-like protein spots appeared at stage Ⅱ (d＜4-5 ㎜). pI value and molecular weight of nomomers of Lb-like protein were narrower and greater than those of soybean, ranging from 4.4 to 5.0 and 15.7 kd respectively. Northern blot hybridization of total RNAs from roots and root nodules using soybean Lb cDNA as a probe made it clear that Lb gene was expressed tissue-specifically only in the root nodules.
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